• 909-548-4900
Ampac USA Logo
  • HOME
  • PRODUCTS
  • INDUSTRIES
  • SOLUTIONS
  • BLOG
  • CONTACT US
Menu
  • HOME
  • PRODUCTS
  • INDUSTRIES
  • SOLUTIONS
  • BLOG
  • CONTACT US
Home Water Filter

Improved Detection of Norovirus and Hepatitis A Virus in Surface Water by Applying Pre-PCR Processing

Sammy Farag by Sammy Farag
June 14, 2018
in Water Filter
0
0
SHARES
0
VIEWS
Share on FacebookShare on Twitter

Borgmastars, E.; Jazi, M. M.; Persson, S.; Jansson, L.; Radstrom, P.; Simonsson, M.; Hedman, J.; Eriksson, R.

Food and Environmental Virology, 9 (4):395-405; 10.1007/s12560-017-9295-32017

Abstract: Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) detection of waterborne RNA viruses generally requires concentration of large water volumes due to low virus levels. A common approach is to use dead-end ultrafiltration followed by precipitation with polyethylene glycol. However, this procedure often leads to the co-concentration of PCR inhibitors that impairs the limit of detection and causes false-negative results. Here, we applied the concept of pre-PCR processing to optimize RT-qPCR detection of norovirus genogroup I (GI), genogroup II (GII), and hepatitis A virus (HAV) in challenging water matrices. The RT-qPCR assay was improved by screening for an inhibitor-tolerant master mix and modifying the primers with twisted intercalating nucleic acid molecules. Additionally, a modified protocol based on chaotropic lysis buffer and magnetic silica bead nucleic acid extraction was developed for complex water matrices. A validation of the modified extraction protocol on surface and drinking waters was performed. At least a 26-fold improvement was seen in the most complex surface water studied. The modified protocol resulted in average recoveries of 33, 13, 8, and 4% for mengovirus, norovirus GI, GII, and HAV, respectively. The modified protocol also improved the limit of detection for norovirus GI and HAV. RT-qPCR inhibition with C q shifts of 1.6, 2.8, and 3.5 for norovirus GI, GII, and HAV, respectively, obtained for the standard nucleic acid extraction were completely eliminated by the modified protocol. The standard nucleic acid extraction method worked well on drinking water with no RT-qPCR inhibition observed and average recoveries of 80, 124, 89, and 32% for mengovirus, norovirus GI, GII, and HAV, respectively.

https://link.springer.com/article/10.1007%2Fs12560-017-9295-3

The post Improved Detection of Norovirus and Hepatitis A Virus in Surface Water by Applying Pre-PCR Processing appeared first on Facts About Water.

Source: Water Feed

Tags: RO
Previous Post

Development of a Cryptosporidium-arsenic multi-risk assessment model for infant formula prepared with tap water in France

Next Post

Distribution System Operational Deficiencies Coincide with Reported Legionnaires’ Disease Clusters in Flint, Michigan

Sammy Farag

Sammy Farag

Next Post

Distribution System Operational Deficiencies Coincide with Reported Legionnaires’ Disease Clusters in Flint, Michigan

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Stay Connected test

  • 23.8k Followers
  • 99 Subscribers
  • Trending
  • Comments
  • Latest
Tips To Hire A Local Plumber To Installing Reverse Osmosis Systems

Tips To Hire A Local Plumber To Installing Reverse Osmosis Systems

November 14, 2022
Reverse Osmosis Pool cleaners as a substitute for chlorine

Reverse Osmosis Pool cleaners as a substitute for chlorine

November 14, 2022
Best Water Neutralization Systems and Products

Best Seawater Desalination- Things You Must Know

March 28, 2023
5 Ways Reverse Osmosis Water Helps Boost Hair Health

5 Ways Reverse Osmosis Water Helps Boost Hair Health

November 14, 2022
RO Water Purification

Oyu Tolgoi Mine – Mongolia

0
Ampac USA Sea Water Desalination Watermakers for the South-Korean Navy

Ampac USA Sea Water Desalination Watermakers for the South-Korean Navy

0
Philippine-Navy

Ampac USA has just Installed a Seawater Desalination System on a Philippine’s New Navy Ship

0

A Global Environmental Solution for Good

0
Solutions For Water Scarcity and Water Hazards

Solutions For Water Scarcity and Water Hazards- What the UN Says About it?

March 28, 2023
Best Water Neutralization Systems and Products

Best Seawater Desalination- Things You Must Know

March 28, 2023
Best Water Neutralization Systems and Products

Best Water Neutralization Systems and Products- Everything You Need to Know About Water Neutralization System

March 28, 2023
Deionized Water Vs. Distilled Water

Deionized Water Vs. Distilled Water- Everything You Need to Know

March 28, 2023

Recent News

Solutions For Water Scarcity and Water Hazards

Solutions For Water Scarcity and Water Hazards- What the UN Says About it?

March 28, 2023
Best Water Neutralization Systems and Products

Best Seawater Desalination- Things You Must Know

March 28, 2023
Best Water Neutralization Systems and Products

Best Water Neutralization Systems and Products- Everything You Need to Know About Water Neutralization System

March 28, 2023
Deionized Water Vs. Distilled Water

Deionized Water Vs. Distilled Water- Everything You Need to Know

March 28, 2023

STAY UP TO DATE BY SIGNING UP FOR OUR WEEKLY NEWSLETTER

  • 5255 State St., Montclair, CA 91763 USA
    Email id: [email protected]
  • US Phone: (909) 548-4900
  • UK: +44(20) 7933-8638
  • Mexico: +52(55) 5350-0716
ABOUT US

Welcome to Ampac USA’s sophisticated water purification systems, which are designed to tackle the most challenging water purification, water supply, wastewater treatment, and seawater desalination challenges in the world’s harshest settings.

KNOW MORE ABOUT

– Industries
– Applications
– Solutions
– Product
– News

INFORMATION LINKS

– About Us
– Privacy Policy
– Disclaimer Policy
– Terms & Conditions
– Delivery Information
– Sitemap

CUSTOMER SUPPORT
– Sign In
– Create Account
– Become a Dealer
– Browser Support
– Orders
Facebook-f Twitter Youtube Linkedin-in

 DMCA.com Protection Status 

Go to mobile version