Facebook X-twitter Youtube Linkedin
  • 909-548-4900
Ampac USA logo
  • Home
  • Products
  • Industries
  • Solutions
  • Contact Us
  • Home
  • Products
  • Industries
  • Solutions
  • Contact Us
Home Water Filter

Comparison of Database Search Methods for the Detection of Legionella Pneumophila in Water Samples Using Metagenomic Analysis

Sammy Farag by Sammy Farag
August 14, 2018
Share on FacebookShare on Twitter

Last updated on April 14th, 2025 at 12:11 pm

Borthong, Jednipit; Omori, Ryosuke; Sugimoto, Chihiro; Suthienkul, Orasa; Nakao, Ryo; Ito, Kimihito

FRONTIERS IN MICROBIOLOGY, 9 10.3389/fmicb.2018.01272 JUN 19 2018

Abstract: Metagenomic analysis has become a powerful tool to analyze bacterial communities in environmental samples. However, the detection of a specific bacterial species using metagenomic analysis remains difficult due to false positive detections of sequences shared between different bacterial species. In this study, 16S rRNA amplicon and shotgun metagenomic analyses were conducted on samples collected along a stream and ponds in the campus of Hokkaido University. We compared different database search methods for bacterial detection by focusing on Legionella pneumophila. In this study, we used L. pneumophila-specific nested PCR as a gold standard to evaluate the results of the metagenomic analysis. Comparison with the results from L. pneumophila-specific nested PCR indicated that a blastn search of shotgun reads against the NCBI-NT database led to false positive results and had problems with specificity. We also found that a blastn search of shotgun reads against a database of the catalase-peroxidase (katB) gene detected L. pneumophila with the highest area under the receiver operating characteristic curve among the tested search methods; indicating that a blastn search against the katB gene database had better diagnostic ability than searches against other databases. Our results suggest that sequence searches targeting long genes specifically associated with the bacterial species of interest is a prerequisite to detecting the bacterial species in environmental samples using metagenomic analyses.

https://www.frontiersin.org/articles/10.3389/fmicb.2018.01272/full

The post Title: Comparison of Database Search Methods for the Detection of Legionella Pneumophila in Water Samples Using Metagenomic Analysis appeared first on Facts About Water.

Source: Water Feed

Tags: RO
Sammy Farag

Sammy Farag

Next Post

Real-Time Online Monitoring for Assessing Removal of Bacteria by Reverse Osmosis

Comments 1

  1. Anonymous says:
    2 weeks ago

    Your article helped me a lot, is there any more related content? Thanks!

    Reply

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Recommended.

Commercial RO System

What are the advantages of Commercial RO Plants?

March 7, 2025
Avoid That Lead – Lead Free Water Strategies, Reverse Osmosis

Avoid That Lead – Lead Free Water Strategies, Reverse Osmosis

August 24, 2019

Subscribe.

Trending.

Which US States Have the Best Water Quality? Get the Answer Here

Which US States Have the Best Water Quality? Get the Answer Here

August 25, 2021
Reverse Osmosis Pool cleaners as a substitute for chlorine

Reverse Osmosis Pool cleaners as a substitute for chlorine

September 19, 2017
Reverse Osmosis system Troubleshooting: Fix 7 Common Problems

Reverse Osmosis system Troubleshooting: Fix 7 Common Problems

December 30, 2024
Water filter guide in USA

Water filter guide in USA

August 5, 2025
Deionized Water Vs. Distilled Water- Everything You Need to Know

Deionized Water Vs. Distilled Water- Everything You Need to Know

January 23, 2023

AMPAC USA 2262 S 1200 W Suite #103 Woods Cross, UT 84087

US Phone: (909) 548-4900

ABOUT US

Welcome to Ampac USA’s sophisticated water purification systems, which are designed to tackle the most challenging water purification, water supply, wastewater treatment, and seawater desalination challenges in the world’s harshest settings.

KNOW MORE ABOUT

  • Home
  • Products
  • Industries
  • Solutions
  • Contact Us
  • Home
  • Products
  • Industries
  • Solutions
  • Contact Us

INFORMATION LINKS

  • About Us
  • Privacy Policy
  • Disclaimer Policy
  • Terms & Conditions
  • Delivery Information
  • Sitemap
  • About Us
  • Privacy Policy
  • Disclaimer Policy
  • Terms & Conditions
  • Delivery Information
  • Sitemap

CUSTOMER SUPPORT

Get updates on special events and receive your first drink on us!

Facebook X-twitter Youtube Linkedin
No Result
View All Result
  • Home
  • Review
  • Apple
  • Applications
  • Computers
  • Gaming
  • Gear
    • Audio
    • Camera
    • Smartphone
  • Microsoft
  • Photography
  • Security

© 2025 JNews - Premium WordPress news & magazine theme by Jegtheme.